Coding

Part:BBa_K3332024:Design

Designed by: Zhichun Yang   Group: iGEM20_XMU-China   (2020-10-17)


phnJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 113
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal NgoMIV site found at 619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 609


Design Notes

Put the one with best activity in the final circuit with phn series, RNAi circuits with best activity and kill switch.


Source

Order the part DNA from Sangon company

References